Searchable abstracts of presentations at key conferences on reproductive biology and medicine

ra0001p245 | (1) | WCRB2014

Study of spermatogonia stem cells from domestic animals: derivation and in vitro culture

Song Hyuk , Lee Won Young , Lee Kyung Hoon

Introduction: It is necessary to establish and culture of spermatogonia stem cells (SSCs) in vitro to perform the meiotic differentiation study and to generate transgenic spermatozoa in vitro or ex vivo. This study was focused on in vitro derivation and establishment of SSCs from porcine and canine.Materials and methods: Five day-old porcine and 2-month-old canine testes were used to isolated SSCs and testicular somati...